General Description of the Volume:
The articles in this Methods in Enzymology volume reflect the integration of molecular genetics with photosynthesis. They focus on the genetic engineering of the photosynthesis apparatus, new mechanisms for genetic selection of photosynthetic mutants, biophysical and biochemical analysis of Photosystem I and Photosystem II Mutations, and Photosynthetic Gene Expression.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Genetic engineering of the photosynthesis apparatus
* New mechanisms for genetic selection of photosynthetic mutants
* Biophysical and biochemical analysis of Photosystem I and Photosystem II Mutations
* Photosynthetic Gene Expression

General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Cell cycle regulators in mammalian systems
* Cell cycle control in yeast and fungal systems
* Analysis of cell cycle regulators in oocyte, egg, and embryonic systems, as well as general methods

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Presents new ideas on classification of proteolytic enzymes
* Covers 100 individual proteolytic enzymes
* Offers numerous medical implications of work in this area
* Provides uses of these enzymes in biotechnology

This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases.
Key Features
* Adenylyl cyclase: determination of activity
* Preparation of materials useful in purification of components of hormonally responsive cyclase systems
* Purification guanine nucleotide-dependent regulatory proteins
* Purification and characterization of g proteins: labeling and quantitating of G proteins
* Reconstitution guanylyl cyclase: assay
* Preparation of materials used for study of the cyclase
* Purification
* Regulation

Many methods have been developed or adapted for the study of RNA processing, which plays a key role in the expression of genetic information. Volumes 180 and 181 of Methods in Enzymology, RNA Processing, Parts A and B, represent the first comprehensive collation of protocols used for the preparation of substrates, for the preparation of extracts and enzymes, and for the analysis of products in RNA processing. At least half of the chapters present techniques that are directly applicable to other areas of nucleic acid biochemistry and cell biology.

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
This volume and its companions (Volumes 330 and 334) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include the enzymes of central metabolism and respiratory enzymes.

This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

General Description of the Volume:
This is the Cumulative Subject Index for Methods in Enzymology Volumes 290-319. It will supplement the Methods in Enzymology Index Volumes 75, 95, 120, 140, 175, 199,299, 265, and 285. In addition to the Subject Index, this volume includes the complete Contents of each volume indexed, and a Contributor's Index.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Preparation of substrates and assay of enzymes
* Cloning, expression, and purification of enzymes
* Detection and consequences of genetic defects
* Regulation and expression of enzymes

General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Total Antioxidant Activity
* Vitamin C
* Polyphenols and Flavanoids
* Thiols
* Vitamin E and Coenzyme Q10
* Carotenoids and Retinoids

General Description of the Volume:
This Methods in Enzymology volume continues the description (initiated in Volume 85) of the purification and assay of this diverse group of proteins involved in cytoplasmic organization. It discusses some of the more promising attempts to apply techniques of molecular biology genetics and molecular cytochemistry to these proteins.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Major topics covered are:
* Analysis of Actinomyosin-Related Systems and of Microtubule-Related Systems
* Other Cytoskeletal Systems
* Cell-Free and Genetic Systems
* Force Production Assays
* General Methods

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Catalysts of Protein Folding: Protein Disulfide Isomerases, Cis-trans Peptidyl Prolyl Isomerases
* Accessory Proteins: Chaperonins, Cochaperonins, Pap Proteins, Sec Proteins
* Physical methods for investigation of interactions between chaperones and their substances
* Cotranslational protein folding, cell-free protein synthesis and associated methods

This cumulative subject index for Methods in Enzymology covers volumes 263, 264, and 266-289. In addition to the Subject Index, this volume includes the complete contents of each volume indexed and a Contributor's Index.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* This volume and its companion Volumes 279, 280, and 281 provide
* A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes
* Details on physical, chemical, and biological properties of vitamins and coenzymes
* Chemical and biological syntheses of vitamins, coenzymes, and their analogs
* Aspects of transport and metabolism of vitamins and coenzymes

Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human diseases and infections. This volume and its companion Volume 288 on Chemokine Receptors provide comprehensive experimental protocols used in this field of research.
Key Features
* C-X-C Chemokines
* C-C and C Chemokines
* Other Methods

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchersin all fields of life sciences.

This volume of Methods in Enzymology and its companion volumes 280, 281, and 282 present new methods and their modifications developed in the past decade plus the more recently acquired knowledge on the functional and metabolic aspects of vitamins and coenzymes. They represent the only in-depth treatment dealing with methods related to vitamins and coenzymes, and contain methods that have never been grouped together before.
Key Features
* This volume and its companion Volumes 280, 281, and 282 provide
* A collation of the most recent and useful methods for the identfication, preparation, and quantification of vitamins and coenzymes
* Details on physical, chemical, and biological properties of vitamins and coenzymes
* Chemical and biological syntheses of vitamins, coenzymes, and their analogs
* Aspects of transport and metabolism of vitamins and coenzymes

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* This volume and its companion Volumes 279, 281, and 282 provide
* A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes
* Details on physical, chemical, and biological properties of vitamins and coenzymes
* Chemical and biological syntheses of vitamins, coenzymes, and their analogs
* Aspects of transport and metabolism of vitamins and coenzymes

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Fluorescence in biophysics
* Fluorescence as a tool for studying proteins
* Determination of ground-state dissociation constant by fluorescence spectroscopy
* Fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions
* Fluorescence methods for studying equilibrium macromolecule-liquid interactions
* Methods for detecting molecular clusters on cell surfaces

Nitric oxide, recently named"Molecule of the Year"by the journal "Science," impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume and its companion Volume 268 highlight methods used to research the physiology and toxicity of nitric oxide in cellular and organismal systems. It will be a valuable resource for the experienced researcher as well as for those newly entering the field.
Key Features
* Effects of nitric oxide in cells and tissues
* Biochemical reactivity
* Tissue-specific effects
* Endothelial nitric oxide synthase expressions in heterologous systems
* Cellular signaling
* Pathological and clinical aspects of NO
* Fingerprints of pathological action
* Interaction with membranes, proteins, and nucleic acids
* Peroxynitrite
* Tissue and cellular cytotoxicity
* Clinical aspects

Nitric oxide, recently named"Molecule of the Year"by the journal "Science," impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume and its companion Volume 269 highlight the most current and up-to-date methods used to research the physiology and toxicity of nitric oxide in cellular and organismal systems. It will be a valuable resourcefor the experienced researcher as well as for those newly entering the field.
Key Features
* Generation, detection, and characterization of biological and chemical sources of nitric oxide
* Biochemistry and molecular biology of enzymes and proteins associated with nitric oxide metabolism

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Liquid chromatography
* Electrophoresis
* Mass spectrometry

General Description of the Volume:
Oncogenes have become a central focus of cancer research. All involved in this discipline will find this volume an invaluable aid in developing protocols needed for their research.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Cell culture
* Molecular cloning
* Gene function
* Protein-protein interactions
* Protein-DNA interactions